Prepared Polyclonal Antibody of the Ciprofloxacin

Abstract

To detect Ciprofloxacin (CPFX) residues in food stuffs of animal origin rapidly by immune method, preparation of high quality polyclonal antibody against CPFX was the most critical step of project. We produced two kinds of antigens for CPFX, using carrier protein bovine serum albumin (BSA) and ovalbumin (OVA) by a modified carbodiimide method, and then the hapten-protein conjugates were characterized by ultraviolet spectrophotometry in detecting qualitatively comparable hapten density, before being used for the immunization and detection purposes. The production of polyclonal antibodies (PcAbs) were sought following the generation of appropriate CPFX-BSA conjugate. One PcAb against Ciprofloxacin (CPFX) was produced and the titer polyclonal antibody could reach 6.25×105. In the optimized ELISA, the PcAb showed 50% inhibition at 10.93 ng/mL for CPFX in buffer.