The Effect of Multispectral Light Emitting Diodes (LEDs) on the Activation of Morphogenic Processes in Cell Culture of Rice Oryza Sativa l

Abstract

The influence of light irradiation with different intensity parameters and different spectral composition on morphogenic structures, namely green zones of cultivar Dolinny cell cultures was investigated. Light irradiation was generated by 10 types of LEDs with different spectra: cold white (Cold W), white (W), warm white (Warm W), Full spectrum, Red, Deep Red, Yellow, Green, Blue and Royal Blue. It was shown that LEDs with Cold W, W and Royal Blue more actively influenced on green zones generation, while Red, Red Deep, Yellow и Green displayed no results. Standard fluorescent lighting illumination (WFL) was used as a control. The number of induced green zones was lower under control luminescent light (WFL) in comparison with the green zones, which were induced by the illumination of the most active spectra of LEDs, but lifetime of these control structures was longer. After 19 weeks of cultivation, degradation of the green zones of the calli was not observed under control and LEDs Cold W, Warm W, and Blue in the position with maximal intensity of light. The Royal Blue illumination displayed the same results not only in the position with maximum intensity but also at the other intensities approaching the control values (49,3 230 μmol/s*m2).