Comparative Studies of Chemical Constituents of Hibiscus rosa-sinensis Using Different Extraction Techniques

Abstract

Hibiscus rosa-sinensis which belongs to the family Malvaceae, is a national flower of Malaysia. This glabrous tree can usually grow around 1 to 3 meters tall. The objective of present study was to categorize the essential oil constituent of flowers and leaves extract using different extraction method namely Soxhlet and hydrodistillation. The essential oil obtained from Soxhlet (10.75%) and hydrodistillation (11.40%) were subjected to Gas Chromatography-Mass Spectrometry (GC-MS) for determination of bioactive compounds. The GC-MS analysis was carried out by Agilent 7980A series GC instrument and DB-1MS capillary column with the dimensions of 30 m × 0.25 mm. A total of 21 and 20 compounds were identified for Soxhlet and hyrodistillation technique, respectively. Major compound found in essential oil for Soxhlet apparatus method was (Z,Z,Z)-9-12,15-Octadecatrienoic acid, methyl ester (23.59%) while for hydrodistillation was 1-Methylene-2b-hydroxymethyl-3,3-dimethyl-4b-(3-methylbut-2-enyl)-cyclohexane (16.57%). The presence of numerous bioactive compounds provides insights to the potential of the Hibiscus rosa-sinensis in pharmaceutical industry.