Antibacterial Activity of Secondary Metabolite Compounds in Ethylacetate Extract of Rumput Mutiara (Hedyotis corymbosa (L.) Lamk)

Abstract

Plant Rumput Mutiara (Hedyotiscorymbosa (L.) Lamk) is one of the family species of Rubiaceae which is used as a traditional medicine that is effective in healing boils, acne, anti-inflammatory, and anticancer. Research methods include maceration, fractionation, purification, class test and bioactivity test with Kirby-Bauer diffusion method using E.coli bacteria. The results of this research are pure isolates with white crystal needle shape with melting point 137-138°C. Pure isolates were analyzed using FTIR, and by the IR spectrum which showed the absorption band at wave numbers 3435.22 cm-1 indicated the presence of –OH, CH3 and-CH2- aliphatic groups (2956.87 cm-1; 2935.66 cm-1; 2893.22 cm-1; and 2866.22 cm-1), C = C (1641.42 cm-1), -CH3 and-CH2- bending (1462.04 cm-1 and 1377.17 cm- 1), CO (1056.99 cm-1), and = CH (964.41 cm-1). Based on the result, it showed that the isolate was steroid group which has low antibacterial activity againts E.coli with inhibition power of 10 mm.