Preparation and Characterization of the Magnetic Layered Double Hydroxide - DNA Delivery System-Reference-Cited by-同舟云学术

Preparation and Characterization of the Magnetic Layered Double Hydroxide - DNA Delivery System

Published:2018-02 Issue: Volume:914 Page:3-10
ISSN:1662-9752
Container-title:Materials Science Forum
language:
Short-container-title:MSF

Author:

Gou Guo Jing¹,Pei Qin Yu¹,Yao Hui Qing¹,Sun Yue¹,Yang Jian Hong¹,Wang Rui¹

Affiliation:

1. Ningxia Medical University

Abstract

The purpose of this paper is to prepare and evaluate a gene delivery system resulted from the intercalation of DNA with magnetic layered double hydroxide (MLDH). The structure and property of the MLDH/DNA hybrids were studied using XRD, FTIR and TG characterization. The cytotoxicity and protection effect of MLDH were evaluated by MTT assay and gel electrophoresis assay. MLDH exhibited lower cytotoxicity than other inorganic nanoparticles. Under physiological conditions, MLDH could protect DNA from enzymatic degradation. Our results confirmed that MLDH can be used safely and effectively as non-viral vectors for gene targeting therapy in form of MLDH/DNA composite.

Publisher

Trans Tech Publications, Ltd.

Subject

Mechanical Engineering,Mechanics of Materials,Condensed Matter Physics,General Materials Science

Link

https://www.scientific.net/MSF.914.3.pdf

Reference27 articles.

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