Hydroxyapatite Growth on Glass/CdSe/SiOx Nanostructures

Abstract

The aim of this study was to find if nanocrystal layers obtained by well-established nanotechnology are able to induce deposition of hydroxyapatite [Ca10(PO4)6(OH)2]. It is known that nanosized objects and porous structures influence biological events and they may be used to create biologically integrated multifunctional devices including biomaterials and sensors. In this work, sequential physical vapour deposition of CdSe and SiO, or SiOx film was used to modify glass substrates. To study the ability of the nanostructured surfaces to induce hydroxyapatite deposition, samples were immersed in a simulated body fluid and simultaneously irradiated with a scanning laser beam for a few minutes. This resulted in a porous sponge-like non homogeneous hydroxyapatite layer, consisting of networks of aggregates of nano dimensions on the modified surfaces. Analysis showed higher Ca and P contents in the stripes of the laser-substrate interaction, which indicated the influence of the laser energy. The method of laser-liquid-solid interaction used has led to a synergistic effect due to the simultaneous use of the nanostructured substrate, aqueous solution and laser energy.