Gold Nanoparticle Based Immunostrip Assay Method for Detection of Protein-A-Reference-Cited by-同舟云学术

Gold Nanoparticle Based Immunostrip Assay Method for Detection of Protein-A

Published:2012-11 Issue: Volume:229-231 Page:260-266
ISSN:1662-7482
Container-title:Applied Mechanics and Materials
language:
Short-container-title:AMM

Author:

Zambre Ajit¹,Chanda Nripen¹,Prayaga Sudhirdas²,Almudhafar Rosana³,Kannan Raghuraman¹,Upendran Anandhi⁴,Afrasiabi Zahra³

Affiliation:

1. University of Missouri

2. Antibody Research Corporation

3. Lincoln University

4. Nanoparticle BioChem. Incorporation

Abstract

We have successfully developed gold nanoparticle based immunostrip assay to detect protein-A (PA). Rabbit polyclonal antibody IGg (αPA) that has affinity to PA was conjugated to gold nanoparticles (GNPs) and the gold nanoconjugate (αPA-GNP) was used to detect protein-A by simple immunostrip assay method. ELISA experiments were used to confirm the retention of binding affinity of antibody towards protein-A after conjugation with gold nanoparticles.

Publisher

Trans Tech Publications, Ltd.

Link

https://www.scientific.net/AMM.229-231.260.pdf

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