The Chronic Toxic Effect of Water-Accommodated Fractions of 0# Diesel Oil on Splox Gene of Sea Urchin-Reference-Cited by-同舟云学术

The Chronic Toxic Effect of Water-Accommodated Fractions of 0# Diesel Oil on Splox Gene of Sea Urchin

Published:2012-12 Issue: Volume:256-259 Page:1998-2003
ISSN:1662-7482
Container-title:Applied Mechanics and Materials
language:
Short-container-title:AMM

Author:

Yang Bai Lin¹,Xiong De Qi¹,Jiang Ling Ling¹,Meng Gang¹

Affiliation:

1. Dalian Maritime University

Abstract

0# diesel is common petroleum hydrocarbon pollutant of sea and marine organisms. One goal of this study was to assess the chronic toxic effect of water-accommodated fractions（WAFS）of 0# diesel oil on Splox gene of Strongylocentyotus intermedius by PCR-SSCP. The experiment was carried out with three concentrations (5mg/L, 20mg/L and 50mg/l). The results show that with the increase of exposure concentration and time, the mutation probability of the sea urchin increased. In the four primers, single base mutation took place in coding region of the 41 of Primer A-1, with the conversion of A-G, where amino acid changed from lysine into arginine.

Publisher

Trans Tech Publications, Ltd.

Link

https://www.scientific.net/AMM.256-259.1998.pdf

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