Determination of Antioxidant Activity In Vitro from Hyptis rhomboidea Mart. et Gal. Ethanol Extracts

Abstract

Antioxidants are the chemical substances that reduce or prevent oxidation. The present study aimed to assess in vitro antioxidant activities of ethanol extracts Hyptis rhomboidea. DPPH, ABTS and FRAP assays were used for characterization of antioxidant activities of several extracts. Eventually, the promising extract was the ethyl acetate (EAc) extract, The analyzed extracts exhibited that antioxidant activities varied considerably as function of extracts. The highest DPPH• scavenging ability was found in Y6-8，which was divided from EAc by silica column, the lowest IC50 value (6.9μg/ml), followed by Y6-9 (11.7μg/ml) and the TEAC of Y6-8 and Y6-9were greater than positive control trolox. The same tendency was observed using ABTS and FRAP assays. These results suggest that the H. rhomboidea shows a better antioxidant activity, especially in the EAc extract and these results would be a guide in the selection of potential cultivars which are used in food and chemical industry and fresh market.