Affiliation:
1. Henan Agricultural University
Abstract
The study on the combined probiotics to replace antibiotics becomes more and more important because the by-effects and appearance of “superbug” from abuse of antibiotics in human life and animal production. In order to get the effective combined probiotics for replacing antibiotics and inhibiting pathogenicEscherichia coli(E. coli) prolification, 3 strains ofBacillus subtilis(B. subtilis, named as B1, B2 and B3) were used to determine the pre-combination with experimental design (3 factors×4 levels), and then the pre-combination ofB. subtiliswas combined withLactobacillus casei(L. casei) andHansenula anomala(H. anomala) to achieve the most effective combined probiotics with the same design. The results indicated that the pre-combination ratio of B1, B2 and B3 was 0:1:1, and the most effective combination ratios of the pre-combination,L. caseiandH. anomalawere 0:1:1, 1:3:0, 2:2:3, 2:3:2, respectively (P<0.05). In addition, the incubating groups containingL. caseihad better effect on inhibitingE. coliprolification than those withoutLactobacillus, indicating thatLactobacillushad the best effect on inhibitingE. coliprolification, compared with other bacteria.
Publisher
Trans Tech Publications, Ltd.
Cited by
9 articles.
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