Study on Synthesis and Characterization of a Fluorescence Probe and its Application for Determination of Superoxide Anion Radical

Abstract

A novel Schiff base, 2-(Salicylideneamino)pyridine (2-SAP) synthesized by 2-aminopyridine and salicylaldehyde was used as a fluorescence probe to determine superoxide anion radical (O2•-) in this study. The synthetic product was characterized by IR, 1H-NMR and GC-MS methods. A fluorescence quenching reaction occurs between 2-SAP and O2•-, and the fluorescence intensity decreased significantly. The effect of pyrogallol concentration (2.0×10-5 M to1.0×10-3 M) was discussed in this study. In the range from 2.0×10-5 M to 1.0×10-4 M, relative fluorescence intensity (y) and pyrogallol concentration (x) shown a good linear relationship, y=37.801x+76.854, r=0.993. Based on this phenomenon, the O2•- generated by pyrogallol autoxidation could be determined simply with high sensitivity.