Biosynthesis of Adenine Arabinoside by Whole Cells of Enterobacter aerogenes HXY2222

Abstract

Adenine Arabinoside were prepared from Ara-U and adenine using whole cells of Enterobacter aerogenes HXY2222. After incubated for 16h, 10% wet cells was added to the reaction mixture, containing 10mM AraU and adenine. The transarabinosylation reaction was carried out at 60°C, in pH 7.5 phosphate buffer, for 30h, and the yield was achieved 83%, based on the molar ratio of both substrates. When the reaction was carried out with 30mM AraU and adenine, the yield of AraA droped significantly due to the low solubility of adeine. 20%~25% (v/v) DMSO were added into the reaction mixture, and a yield of 77% was achieved.