Affiliation:
1. Chinese Academy of Sciences
2. Jinan University
Abstract
In order to determination of cholesterol in human serum with no reagent using near-infrared (NIR) spectroscopy. Interval partial least square (iPLS) was proposed as an effective variable selection approach for multivariate calibration. For this purpose, an independent sample set was employed to evaluate the prediction ability of the resulting model. The spectrum was split into different interval. Then, the informative region of cholesterol (1688-1760nm), in which the PLS model has a low RMSEP with 0.241mmol/L and a high R with 0.975, is selected with 23 intervals. The results indicate that, the informative region of cholesterol can be obtained by iPLS and applied to design the simpler reagentless NIR instruments for inexpensive cholesterol measurement in future.
Publisher
Trans Tech Publications, Ltd.
Reference9 articles.
1. K. Jeon, S.J. Kim, K.K. Park, J.W. Kim, and G. Yoon, Noninvasive total hemoglobin measurement,. Journal of Biomedical Optics, vol. 7, pp.45-50, (2002).
2. W. Pothisarn, A non-invasive hemoglobin measurement based pulse oximetry,. SPIE, pp.4916-4982, (2002).
3. Y. Koshik, and Y. Yamaroshi, Pulse glucometry: a new approach for noninvasive blood glucose measurement using instantaneous differential near-infrared spectrophotometry,. Journal of Biomedical Optics, vol. 11, p.054028, (2006).
4. K. Norris. Possible medical applications of NIR,. Making light work: Advances in near infrared spectroscopy, Edited by I. Murray & I.A. Cowe. U K: Ian Michael Publication, pp.596-602, (1992).
5. N. Kang, S. Kasemasumran, Y.A. Woo, H.J. Kim, and Y. Ozaki, Optimization of informative spectral regions for the quantification of cholesterol, glucose and urea in control serum solutions using searching combination moving window partial least squares regression method with near infrared spectroscopy, Chemom. Intell. Lab. Syst., vol. 82, p.90–96, (2006).