Study on Zostera marina L. Seed Germination Ecological Characteristics in Variable Environmental

Abstract

The paper is mainly concerned with two parts in morphological observation and tissue culture of Eelgrass (Zostera marina L.).The results areas follows: 1.The microstructure of the plant showed that the cell wall exposing to seawater in epidermal cells of leaves was highly thickened, but the other sides were normal. It was also highly thickened in the side exposing to seawater in the epidermal cells of roots and stems. This structure may function as a barrier against toxic ions to enter the epidermal cells. Chloroplasts were found mainly in the epidermal cells, indicating that epidermal cells were main site where photosynthesis occurred. Aerenchyma was found everywhere in the plant which showed the ability of adaptation to submerged life. Anatomical observation of the inflorescence showed that eelgrass was a monoecious plant; the male flower and carpel were alternating arrangement on the inflorescence. SEM photographs of the stamen and the carpel indicated that the carpel had two stigmas; the pollens of the eelgrass were filamentous. All of the above observation showed that eelgrass is able to adapt to submerged life. The explants were dipped in 75%Alcohol for 10 seconds firstly, they were dipped in 1%NaClO 10 minutes, and this was the best way of sterilization than other treatments.