Study on Cytotoxicity of “Dextran-Magnetic Layered Double Hydroxide–Fluorouracil” Targeted Liposomes In Vitro

Abstract

This paper studied the cytotoxicity of the dextran-magnetic layered double hydroxide-fluorouracil targeted liposomes ( DET-MLDH-FU liposomes, DMFL ) by MTT method against human ovarian adenocarcinoma cancer cells ( SKOV3 )in vitro. The results shown that the cellular inhibition rate at 24 h increased with the increasing of the fluorouracil concentration in DMFL, DET-MLDH-FU ( DMF ), fluorouracil-loaded liposomes ( FUL ) and free fluorouracil ( FU ). The order of inhibition rate was DMFL, DMF, FUL and FU at same concentration and intervention time. The IC50values of DMFL, DMF, FUL and FU were 14.39, 25.47, 34.39, 48.71 ( μgmL-1), respectively. The results of cytotoxicity experiment proved that the DMFL was superior than other preparations of fluorouracil.