Application of Magnetic/Magnetic Fluorescent Microsphere Preparation in Genes Food Testing

Abstract

Polymerase chain reaction (PCR) and fluorescence quantitative polymerase chain reaction (qRT - PCR) is a major means of detection of genetically modified food, whose key is to extract high quality nucleic acid in quick and high flux. The main nucleic acid extraction method is hexadecyl trimethyl ammonium bromide (CTAB) and sodium dodecyl sulfate (SDS) and kits. Because operation of CTAB and SDS method are cumbersome, require the use of organic solvent and centrifugal unceasingly, thus can not meet the requirement of the rapid development of high throughput; and because of the high price of kit method, its application in the daily inspection is also limited. At the same time, the development trend of genetically modified detection based on nucleic acid level is multiple tests; the key is the preparation of different kinds of fluorescent microspheres and specific probe of microspheres. To solve above problems, this paper put forward nucleic acid extraction method on the basis of the magnetic separation technology.