Inhibition of TGFα-Saporin on Neointimal Hyperplasia via Inhibiting Smooth Muscle Cells Proliferation and Promoting its Apoptosis

Abstract

Objective: To investigate the effects of TGFα-SAP on the inhibition of neointimal hyperplasia of rat which suffered from common carotid arterial injury. Methods: Saporin and TGFα were conjugated by using N-succinimidyl-3 (2-pyridyldithio) propionate. seventy rats were divided into two groups at random, the TGFα-SAP treated group was treated with local injection of TGFα-SAP (5μg/kg) after injury, and the control group was treated with saline. PCNA analysis and TUNEL analysis were performed. Results: Compared to the control group, the TGFα-SAP treated group showed a significant inhibition of intimal thickness and exhibited a lower P/A ratio and a higher rate of apoptosis. Conclusion: TGFα-SAP, as a potential stent coating material, could inhibit neointimal hyperplasia by inhibiting the proliferation and promoting the apoptosis of smooth muscle cells in the neointima.