Bioremediation of Biodiesel/Diesel Blend (B50) in Soil Contamination by Using Pseudomonas putida

Abstract

The biodiesel fuels nowadays are attracting worldwide attention as blending components or direct replacements for diesel fuel in conventional diesel engines. This study aims to investigate the effectiveness ofPseudomonas putidaas oil-biodegradable agent in soil contaminated with biodiesel/diesel blends (B50). The effectiveness on bioremediation has been conducted by examining several physico-chemical tests on biodiesel/diesel-contaminated soil before and after seeding ofPseudomonasputida. The spillage stimulation of B50 was conducted at laboratory scale for 24 days of incubation time. The results show that the bioremediation treatment able to remove up to 82%, 54%, 38% and 10% of nitrogen, sulfate, phosphate and organic carbon, respectively. The pH of soil sample was changed from pH 7.42 (Day 0) to pH 6.38 (Day 24) after the treatment. Meanwhile, the moisture content in the sample has increased from 25.61% (Day 0) to 31.83% (Day 24). All results show the good indication of quality improvement for polluted soil after treated withPseudomonasputida. It is apparent from the acquired results that the application ofPseudomonasputidais suitable as effective microorganism and potentially useful as oil-soil biodegradable agent in polluted soil.