Bioremediation of Biofuel-Soil Contamination by Using Pseudomonas putida

Abstract

Abstract. The commercialization of biodiesel/diesel blends on the market can cause environmental damages due to spills. This study aims to investigate the effectiveness ofPseudomonas putidaas oil-biodegradable agent in soil contaminated with biodiesel/diesel blend (B20). The effectiveness on bioremediation have been conducted by examined several physico-chemical tests on biodiesel/diesel-contaminated soil before and after seeding ofP. putida. The spillage stimulation of B20 was conducted at laboratory scale for 24 days of incubation time. The results show that the bioremediation treatment able to remove up to 82%, 77%, 16%, and 10% of nitrogen, phosphate, sulfate and total organic carbon, respectively. The pH of soil sample was changed from pH 7.45 (Day 0) to 7.25 (Day 24) after the treatment. Meanwhile, the moisture content in the sample has increased from 44.11% (Day 0) to 50.35% (Day 24). All of these results show the good indication of quality improvement of polluted soil after treated withP. putida. It is apparent from the acquired results that the application ofP. putidais suitable as effective microorganism and potentially exploits as useful oil-soil biodegradable agent in polluted soil.