The Isolation of a Pseudomonas aeruginosa Strain and the Effects on the Degradation of Malachite Green and its Enzymatic Mechanism

Abstract

A strain isolated from activative sludge, on microbial degradation of malachite green and its enzymatic mechanism was studied. The isolation of microorganism, malachite green degradation and enzyme activity assay experiment, was determined the effects of microbial degradation of malachite green and its mechanism. The 16SrDNA gene sequence of a malachite degrading bacterial isolated was 99.8% similar to Pseudomonas aeruginosa AS2 and identified as Pseudomonas aeruginosa. The P. aeruginosa strain could degrade malachite green in the 4000 mg.L-1, and its degrading volcity was positively related to PPO and CMCase activities. The isolation has ability on degrading of high concentration of malachite green.