Affiliation:
1. Shenyang Agricultural University
Abstract
First of all, whey protein concentrat was added to milk adjusting the proportion of casein and whey proteins to 40:60. And then milk was hydrolyzed by trypsin and flavourzyme (TF) single respectively in one-step process or staged in two-step process. The Antigen contents of α-lactalbumin (α-LA) and β-lactoglobulin (β-LG) during the hydrolysis process were determined by indirect competitive enzyme-linked immunosorbent assay (ELISA) methods. The result showed that the most significant antigen reduction was observed in two-step hydrolysis process compared with one-step hydrolysis process
Publisher
Trans Tech Publications, Ltd.