Micropropagation of Highly Productive Forms of Diploid and Triploid Aspen

Abstract

The stages of getting the planting material for laying plantations of the triploid aspen (Populustremula L.) are illustrated in this work. The source material selected from the genetic fund “The gigantic aspens” is characterized. The peculiarities of using the method of clonal micropropagation when getting the planting material, the usage of a rootstock as a source of donor explants for the introduction into the culture in vitro, the influence of a kind of nutrient media and growth regulators NAA and BAP on the growth and the development of microsprouts. The distinctions in the speed of the growth of the culture in vitro of diploid and triploid clones have been analyzed. The adaptation of the plants-regenerants to the soil-conditions.