Determination of Pentachlorophenol and Sodium Pentachlorophenolate in Fishery Products by Acetic Anhydride Derivatization/GC/μ-ECD

Abstract

A method was estimated to detect pentachlorophenol and sodium pentachlorophenolate in fishery products by gas chromatography with μ-ECD detector. Procedures of the method included extraction, alkaline stripping and derivatization. The pH of sample matrix was modified to 3-4 by nitric acid solution(nitric acid:water 1:1). Target compounds were extracted by hexane first and 0.2mol/L potassium hydroxide solution was used as stripping reagent to isolate pentachlorophenol from hexane. Acetic anhydride was taken as derivatizing reagent to convert target compounds into nonpolar ester compounds according to acylation reaction. Derivatized compound was extracted by hexane for the analysis of GC. The limit of detection is 2µg/kg . The average recoveries ranged from 73.01 to 104.68% spiked at 2.0,4.0,10.0µg/kg. And the relative standard deviations ranged from1.5 to 8.3%. The method can be used for the determination of pentachlorophenol and sodium pentachlorophenolate in fishery products,and it provides an rapid and acute method for food safety determination.