Molecular Cloning, Association of Bovine α-Actinin1 Gene Variant with the Reproduction Traits

Abstract

To study the relation between α-actinin1 gene and reproduction traits, 442 different varieties cattle were used as experimental populations. PCR-RFLP and DNA sequencing was used to detect the SNP and genotype analysis in these populations. RT-PCR technique was used to isolate cDNA of bovine α-actinin1gene. The association analysis of this ApaⅠ locus polymorphism with reproduction traits were analyzed by using GLM procedure. The result showed that the mutation of G/A,using ApaⅠ endonuclease, was detected in 10th intron of at the α-actinin1 gene locus. The mutation caused ApaⅠpolymorphism in group, there were α-actinin1-G and α-actinin1-A genotypes.The statistical analysis of α-actinin1 163-SNP with reproduction traits showed that there was no associated with the number of calving.The sequence of cDNA of bovine α-actinin1 was 2 911bp. It contained an open reading frame of 2 679 bases (from 53bp to 2 731bp) which encoded an 892 amino acid.