Detection to E. Coli O157 by Surface Plasmon Resonance Immunosensor

Abstract

This work describes an approach for the development of two bacteria biosensors based on surface plasmon resonance (SPR) technique. The first biosensor was based on functionalized gold substrate and the second one on immobilized silver nanoparticles. For the first biosensor, the gold substrate was functionalized with acid–thiol using the self-assembled monolayer technique, while the second one was functionalized with silver nanoparticles immobilized on modified gold substrate. A polyclonal anti-Escherichia coli antibody was immobilized for specific (E. coli) bacteria detection. Detection limit with a good reproducibility of 104 and 103 cfu•mL−1 of E. coli bacteria has been obtained for the first biosensor and for the second one respectively.