Systematic Evaluation of Hybridization Property and Photo-Crosslinking Ability of Modified Oligonucleotides Containing Benzophenone-Bearing Glycol Nucleoside Analogs

Abstract

Antigene technology is the one strategy for the artificial regulation of gene expression by the formation of triple structure on triplex forming oligonucleotide (TFO) with dsDNA. For the enhancement of the thermal stability of triplex structure, photo-crosslinking reaction is attractive by the covalent bond formation between TFO and dsDNA. In this paper, we designed the novel TFOs containing benzophenone moiety as a photo-crosslinkable agent. Several types of glycol nucleoside analogs having glycol scaffold and benzophenone residues with different linker length were prepared, and the these were incorporated into TFOs. The thermal stability of triplex and the photo-crosslinking reaction efficiency of TFOs toward dsDNA was systematically evaluated.