Affiliation:
1. Songkhla Rajabhat University
Abstract
Benzoic acid analysis in curry paste samples were carried out by using high performance liquid chromatography using ultrasonic extraction. Methanol-0.05 M ammonium acetate buffer pH 4.40 in the ratio of 55:45 (%v/v) at a flow rate of 1.00 mL/min was used as the mobile phase and benzoic acid detection was performed at 226 nm using an UV-Visible detector. Under the optimum conditions, linearity of spiked samples ranged from 50 to 3,000 mg/kg. Matrix matched calibrations had determined that benzoic acid contents in southern sour, red, and green curry paste samples were 67.59, 78.62 and 72.33 mg/kg, respectively. Recoveries were obtained from 89.34 to 101.70%, 83.37 to 130.30% and 92.75 to 113.56% with R.S.D. ranged from 2.71 to 6.53%, 4.02 to 11.58% and 5.81 to 6.35%, for southern sour, red, and green curry paste samples, respectively.
Publisher
Trans Tech Publications, Ltd.
Reference13 articles.
1. WHO, Concise International Chemical Assessment Document; 26: Benzoic acid and sodium benzoate, World Health Organization, Geneva, 2000. Information on http://www.who.int/ipcs/ publications/cicad/cicad26_rev_1.pdf.
2. J. Burana-osot, L. Arunsingkharat, M. Naksuk, S. Naungnamjai, T. Saetun, Validation of a HPLC Method for the Determination of Benzoic Acid and Sorbic Acid in Noodles, Chiang Mai J. Sci. 41(2) (2014) 370-382.
3. CAC, Recommended methods of analysis and sampling (Codex STAN 234-1999), Codex Alimentarius Commission, Rome, 1999. Information on http://www.fao.org/fao-who-codexalimentarius/standards/list-standards/en.
4. R. Wood, L. Foster, A. Damant, P. Key, Analytical methods for food additives, Woodhead, Cambridge, (2004).
5. Z.-H. Wang, J.-F.Xia, F.-Y.Zhao, Q. Han, X.-M.Guo, H. Wang, M.-Y. Ding, Determination of benzoic acid in milk by solid-phase extraction and ion chromatography with conductivity detection, Chi. Chem. Lett. 24 (2013) 243-245.