Automatic Egg-Cell Gathering by Two Versatile Miniature Robots and a Miniature Pump on an Inverted Microscopy

Author:

Fuchiwaki Ohmi1,Ichikawa Tatsuya1,Yamagiwa Tsubasa1

Affiliation:

1. Yokohama National University

Abstract

In this paper, we have described automatic gathering with visual feedback of egg-cells by 2 miniature robots. Generating a transgenic mouse is one of the most important operations in the bio-medical field. Sometimes scientists need several hundreds of egg-cells for only 1 experiment. However, conventional manipulators are designed for only DNA-injecting, and they are not useful devices for gathering egg-cells. To support the DNA-injecting operation, we have proposed the automatic gathering of egg-cells by 2 miniature robots and a miniature pump. In several experiments, we have checked the efficiency and confirmed that our device has good feasibility to support egg-cell processing. We have also proposed a 5 pipettes’ egg-cell processing method using miniature robots to realize multi-functional and flexible egg-cell processing.

Publisher

Trans Tech Publications, Ltd.

Subject

Mechanical Engineering,Mechanics of Materials,General Materials Science

Reference10 articles.

1. http: /narishige-group. com.

2. http: /www. eppendorf. com.

3. Y. Sun, B.J. Nelson: Autonomous injection of biological cells using visual servoing, Experimental Robotics VII, eds. D. Rus and S. Singh, Springer-Verlag London Ltd., pp.169-178, (2001).

4. T. Trüper, A. Kortschack, M. Jähnisch, H. Hülsen, S. Fatikow: Transporting cells with mobile microrobots, IEEE Proc. -Nanobiotechnology, Vol. 151, No. 4, pp.145-150, (2004).

5. H. Hülsen, T. Trüper, S. Fatikow: Control System for the automatic Handling of biological Cells with mobile Microrobots, Proc. American Control Conference (ACC'04), Boston, MA, U.S.A., pp.3986-3991, (2004).

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