Alpha-Glucosidase Immobilization Based on PMMA/Chitosan Core-Shell Microparticles

Abstract

Objective To obtain immobilized alpha-glucosidase with good biological activity and improve the utilization rate of alpha-glucosidase. Methods Prepare the core-shell microparticles consisting of poly (methyl methacrylate) (PMMA) cores surrounded by various of chitosan shells, induced by tert-butylhydroperoxide (TBHP). Then fixed the alpha-glucosides N-terminal onto the carriers and studied the optimum immobilization conditions and the property of alpha-glucosidase immobilized.Result Immobilized α-glucosidase enzyme pH stability was higher than the free. In particular, the relative enzyme activity were maintained at 80% in the range of pH4.5 ~ 6.5. Immobilized α-glucosidase optimum temperature is 60 °C, the optimum temperature of the free enzyme is 50 °C. Conclution These Alpha-glucosidase Immobilization can be used as biopolymer and biomaterials in the pharmaceutical and medical application fields.