Abstract
Investigation on the antifeedant activity and toxicity of Catunaregam Spinosa (Rubiaceae)fruits revealed that the n-butanol fraction from methanolic extractof Catunaregam Spinosa fruits showed effective antifeedant activity and toxicity against the larvae of Pieris rapae and Plutella xylostella. Continued chemical investigation on the n-butanol fraction yielded five triterpenoids. Use of NMR, MS and sugar analysis gave structures of three triterpenoid saponins with branched monosaccharide chain as 3-O-[β-D-glucopyranosyl-(1→3)-β-D-6-O-methyl-glucuronopyranosyl oxy]-2β-hydroxy-olean-12-en-28-oic acid (1), 3-O-[β-D-glucopyranosyl-(1→2)-β-D-glucopyran -osyl]-olean-12-en-28-oic acid (2), 3-O-[β-L-rhamnopyranosyl-(1→3)-β-D-glucuronopyranosyl- (1→2)-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl]-12-en-28-oic acid (3), and along with triterpenoids as Oleanolic acid (4), 3β,23-dihydroxy-olean-12-ene-28-oic acid (5), respectively.
Publisher
Trans Tech Publications, Ltd.
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