Affiliation:
1. Huazhong Agriculture University
Abstract
The fusion solution is a significant factor for the effect of the cell electrofusion, traditional buffer solution is different from the cell culture solution, researchers would spend lots of time and money to move the cells from the culture solution (DMEM) into the fusion buffer. The aim of this study is to explore a new formula on the basis of DMEM solution instead of Zimmerman solution. Theoretically its feasible to instead of fusion solution when the ratio of DMEM solution and water is less than 9:500.
Publisher
Trans Tech Publications, Ltd.