Affiliation:
1. Jiangxi University of Traditional Chinese Medicine
2. Chengdu University of TCM
3. Henan Wan Xi Pharmaceutical Co.,Ltd
Abstract
Prepared Tanshinone IIA lipid microspere and evaluated its physicochemical properties in vitro and pharmacodynamics properties in vivo. Particle size, zeta potential, entrapment efficiency, quantitative determination and stability of Tan-IIA-LM with different dilution medium were investigated respectively by Dynamic Light Scattering, HPLC and Sephadex G-50 Microcolumn, and osmotic pressure and solution pH of Tan-IIA-LM were measured to evaluate the preparation comprehensively. Further more, comparative study of protection on focal cerebral ischemia/reperfusion injury in rats between Tan-IIA-LM and STS was studied.The results showed that average particle size was 203nm, zeta potential was-36.10 mV, content was 0.8mg·mL-1, entrapment efficiency was 93% and the Tan-IIA-LM which was diluted by 0.9% sodium chloride injection and 5.0% glucose injection were stable within 6 hours. Tan-IIA-LM had obvious protection on focal cerebral ischemia/reperfusion injury in rats.
Publisher
Trans Tech Publications, Ltd.
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