Protective Effects of Quercetin Derivatives against Hydrogen Peroxide-Induced Oxidative Damage in PC-12 Cells

Abstract

In order to search for new structural modification strategies on quercetin, we have designed and synthesized a series of quercetin derivatives. Several novel quercetin derivatives displayed potent protect effect on H2O2-induced injury in PC12 cells. In the recent study, we investigated the effect of 3,3',4',5,7-pentahydroxyflavone 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one (HPS5) on the apoptosis of PC12 cells in vitro. We used neuronal PC12 cell line to study the regulation role of HPS5 in H2O2 induced cell apoptosis. MTT assay was used for detection the proliferation of PC12 cells upon H2O2 treatment. PC12 cell nuclear morphological change was detected with Hoechst 33258 staining. The antioxidative capacity was judged by SOD determinations. The protein levels of Bax and Bcl-2 were deteanined by Western blotting. Our results demonstrated that Quercetin derivatives HPS5 can prevent and protect against H2O2-induced injury in PC12 cells. Together, our studies provided new insights activities of quercetin, and may contribute to pharmacological study of quercetin in future research.