A Method for the Determination of Atropine in Pharmaceutical Preparation Using CE-ECL

Abstract

In this paper, a sensitive determination method for atropine based on end-column electrochemiluminescence of tris (2,2’-bipyridyl) ruthenium (II) detection is described. The conditions affecting separation and detection were investigated. Favorable ECL intensity of atropine was achieved in a solution consisting 5 mmol/L Ru (bpy)32+ and 50 mmol/L phosphate at applied voltage of 1.20V. The standard curve was linear between 1 and 20 μmol/L for atropine. The calibration equation and regression coefficients were: y = 128.38x−36.76 and R = 0.998 in terms of peak height response as a function of analyte concentration. A detection limit of 5×10−8 mol/L (S/N= 3) was achieved. The proposed method was applied satisfactorily to the determination of atropine in three pharmaceutical preparations.