Chemical Constituents and Antiamoebic of Methanolic Fraction from Peperomia pellucida (Linn.)Kunth

Abstract

Acanthamoeba spp. is the free living protozoain the environment which are opportunistic pathogens of human [1]. Acanthamoeba spp. is the causative agents of Acanthamoeba keratitis (AK)[2]. The risks of AK increased by using contact lens are not good hygiene. Antiamoebic therapy were biguanides chlorhexidine (BCH) or polyhexamethylene biguanide (PHMB) which were not effective enough to kill Acanthamoeba cysts [3]. Reported, plant for protected glaucoma, anti-inflammatory and analgesic properties are Peperomiapellucida in Piperaceae family. This interesting of studied P. pellucida for antiamoebic. Dried plant of P. pellucida was chromatographic by Diaion HP20 and fractionated into methanol : water (1:1, FrI), methanol (FrII) and ethyl acetate (FrIII). The methanol fraction (FrII) was damaged morphological and changed structural of Acanthamoeba cysts, detected by toluidine dye staining are shown under the light microscope, in IC50 29.28±3.64%.The active methanol fraction was studied by chromatography, gave four fractionated fraction A1-A4. A1 led to the isolation of compounds 1, A2 and A3 isolated compounds 2-5. They were identified using spectroscopic methods.