Identification of Tocopherols from Rhodiola sachalinensis by Using GC/MS

Abstract

The tocopherols inRhodiola sachalinensiswere determined with adsorption column chromatographic purification and GC/MS. Tocopherols were extracted by sonication and Soxhlet with ethanol and dichloromethane, respectively. The extract was partitioned with chloroform and water using liquid-liquid extraction, and water part was partitioned with ethyl acetate, and purified with a silica column. Three type of tocopherols such as α, β, γ-tocopherol were identificated fromRhodiola sachalinensisby GC/MS. All of the three types of tocopherols were determined in the chloroform part, with some sterol and sterol conjugates. The similarities of the three types of tocopherols were nearly or above 90%, and the retention time of α, β, γ-tocopherols were 24.085, 23.194, and 22.458min, respectively.