Isolation and Identification of Hyperoside in <i>Abelmoschus manihot</i> Flower-Reference-Cited by-同舟云学术

Isolation and Identification of Hyperoside in Abelmoschus manihot Flower

Published:2013-09 Issue: Volume:781-784 Page:880-883
ISSN:1662-8985
Container-title:Advanced Materials Research
language:
Short-container-title:AMR

Author:

Qiu Yan¹,Wang Ming Zhu²

Affiliation:

1. Hebei Uiversity of Science and Technology

2. Hebei University of Science and Technology

Abstract

Abelmoschus Manihot (L.) Medic flowers are traditionally used for food and medicinal materials. With the aim of acquiring the best method of isolating hyperoside from total flavone in A. Manihot flowers, this study investigated the effects of different eluted factors on separating hyperoside in total flavone of crude extracts. Gradient elution was used to separate the total flavone of ethanol extraction. The eluates were isolated by silica gel thin-layer chromatography (TLC) and identified by HPLC. The results showed that the elution components (chloroform: methanol = 50: 50) isolated by TLC (developing agent ethyl acetate: formic acid: water = 8:3:1) presented a clear light yellow stripe which was identified as hyperoside by HPLC.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

Link

https://www.scientific.net/AMR.781-784.880.pdf

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