Advancement in Correlation Study between Cell Apoptotic Stages and its Morphological Variations via Single-Cell Techniques

Abstract

In recent years, the appreciation of cellular heterogeneity instead of cell information inferred from population-level data has driven the development of single-cell techniques. Among those adopted in this research were AFM characterization, fluorescence technique and a self-developed cell-targeting process in order to correlate precisely the cells early or late apoptotic stage with its corresponding morphology status, the membrane roughness in this paper. Research results were compared to results revealed from our previous research for the effectiveness verification of the proposed scheme.