Ultrasound-Microwave Assisted Extraction and Separation of <i>Ricinus communis</i> Allergen from Castor Bean Meal-Reference-Cited by-同舟云学术

Ultrasound-Microwave Assisted Extraction and Separation of Ricinus communis Allergen from Castor Bean Meal

Published:2013-09 Issue: Volume:781-784 Page:721-725
ISSN:1662-8985
Container-title:Advanced Materials Research
language:
Short-container-title:AMR

Author:

Zhang Ai Lin¹,Wang Chang Lu¹,Hu Yu Feng¹,Zhou Zhi Jiang²

Affiliation:

1. Tianjin Engineering Center of Food Process

2. Tianjin University

Abstract

The influence of ultrasound-microwave during extraction of Ricinus communis(L）allergen from castor bean meal (CBM) was investigated. The method was successfully applied for the ultrasound- microwave assisted extraction (UME) of allergen from CBM. The corresponding extraction parameters including the extraction temperature, ultrasonic-microwave power and extraction time were investigated. The CBM was crushed, sifted, ground and suspended for 4 h in 25% ethanol water (1:4) at room temperature followed by ultrasound-microwave. The yield, polysaccharose and protein contents were determined after each processing step. The purity of extracted allergen was tested through HPLC and the Sephadex G-100 and Tricine-SDS-PAGE detection with unique electrophoresis strip documented that allergen was purifed.. The paper describes the conditions of the temperature,time,and microwave-power for the method reliability with UME and suitablilty for the effective extraction of allergen from CBM.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

Link

https://www.scientific.net/AMR.781-784.721.pdf

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