Affiliation:
1. Chinese Academy of Fishery Sciences
Abstract
A high performance liquid chromatography-tandem mass spectrometric method had been established for simultaneous determination of 25 sex hormones. The hormones were extracted by ethyl acetate and ethyl acetate-tert-butyl methyl ether (1/1, v/v) after enzymatic hydrolysis, followed by an LC-C18 cartridge for cleanup, then were quantified by HPLC-ESI MS/MS with internal standards. Limits of quantification were 1-2 μg.kg-1, average recoveries were 92-113 %, and the R.S.D. was 6-10 %.
Publisher
Trans Tech Publications, Ltd.
Reference14 articles.
1. H. Noppea, B. L. Bizec, K. Verheyden: Anal. Chim. Acta. Vol. 611 (2008), pp.1-16.
2. S. Hartmann, H. Steinhart: J. Chromatogr. B. Vol. 704 (1997), pp.105-117.
3. Hsin-Chang Chen, Han-Wen Kuo, Wang-Hsien Ding: Chemosphere. Vol. 74 (2009), pp.508-514.
4. A. Vermeulen, J.P. Deslypere, R. Paridaens: J. Cancer Res. Clin.
5. Li-Qiang Qin, Pei-Yu Wang, Takashi Kaneko: Med. Hypotheses. Vol. 62 (2004), pp.133-142.
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