Affiliation:
1. National Institute of Health Sciences
2. Kyoto University
Abstract
Accurate measurement of the mechanical properties of artificial or cultivated cartilage is
a major factor for determining successive regeneration of defective soft tissues. In this study, we
developed a novel method that enabled the bulk modulus (k-modulus) to be measured
nondestructively using the relationship between volume and pressure of living soft tissues. In order
to validate this method we estimated the bulk modulus of soft silicone rubbers using our new
method and a conventional method. The results showed a 5 ~ 10% difference between the results
obtained with the two methods. Our method was used subsequently to measure the mechanical
properties of cultivated cartilage samples (collagen gel type), that had been incubated for four
weeks in the presence or absence of human articular chondrocytes (HACs). Our experiments
showed that cultivated cartilage tissues grown in the presence of HACs had a higher bulk modulus
(120 ± 20 kPa) than samples grown without HACs (90 ± 15 kPa). The results indicated that our
novel method offered an effective method for measurement of volume changes in minute living soft
tissues, with the measurements having a high degree of accuracy and precision. Furthermore, this
method has significant advantages over conventional approaches as it can be used to rapidly and
accurately evaluate the strength of soft tissues during cultivation without causing damage to the
specimen.
Publisher
Trans Tech Publications, Ltd.
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science
Cited by
1 articles.
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