Effect of Rapamycin/Curcumin Co-Loaded Poly(Lactide-Co-Glycolide) Films on Blood Compatibility and Vascular Smooth Muscle Cell Proliferation

Abstract

Rapamycin/curcumin co-loaded and rapamycin-loaded poly(lactide-co-glycolide)(PLGA) films were prepared by a casting method. The components of different drug-loaded films were analyzed by Fourier transform infrared spectroscopy (FTIR), and the major peaks of rapacymin and curcumin were both observed in these films. Compared to the rapamycin-loaded film, the results of in vitro platelet adhesion tests show that the number of adhered platelets reduce, and few aggregated and activated platelets are observed. The APTTs (activated partial thromboplastin time) of rapamycin/curcumin co-loaded films is nearly 6 seconds longer than for the rapamycin-loaded PLGA films. All results indicate that curcumin may suppress thrombosis activated by rapamycinloaded film, and improve the anticoagulative property. The results of alamar blue indicate that the rapamycin/curcumin co-loaded film has better antiproliferation effect than 316 stainless steel (SS).