Attachment and Proliferation of Human Periodontal Ligament Fibroblasts on Fibronectin-Coated Bioactive Glass Modified Ceramics

Abstract

The effect of fibronectin (FN) on human periodontal ligament fibroblasts (PDLF) attachment and proliferation on Bioglass® (PerioGlas® Synthetic Bone Graft Particulate, US Biomaterials) modified dental ceramics, was investigated in vitro. FN introduced limited alterations in cell attachment on Bioglass®-modified dental ceramics in comparison with the corresponding non-FN-coated specimens but had a profound positive effect on Bioglass®-coated specimens that weakly supported both cell attachment and proliferation. The amount of protein adsorbed on the specimens was not proportional to its biological activity, i.e. cell attachment, spread and proliferation, probably due to surface energy variations and FN conformational changes induced by differences in surface composition and morphology of the different dental ceramics modifications.