The Effect Orthosilicic Acid on Collagen Type I, Alkaline Phosphatase and Osteocalcin mRNA Expression in Human Bone-Derived Osteoblasts In Vitro-Reference-Cited by-同舟云学术

The Effect Orthosilicic Acid on Collagen Type I, Alkaline Phosphatase and Osteocalcin mRNA Expression in Human Bone-Derived Osteoblasts In Vitro

Published:2006-05 Issue: Volume:309-311 Page:121-124
ISSN:1662-9795
Container-title:Key Engineering Materials
language:
Short-container-title:KEM

Author:

Arumugam Meera Q.¹,Ireland D.C.¹,Brooks Roger A.²,Rushton Neil²,Bonfield William²

Affiliation:

1. Cambridge University

2. University of Cambridge

Abstract

The object of this study was to investigate the effect of the concentration of orthosilicic acid (0, 0.5, 1, 5 and 10µM) on gene expression in human osteoblast cells isolated from trabecular bone. This was measured using reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify messenger RNA (mRNA) levels for collagen type I, alkaline phosphatase and osteocalcin. Results showed that while collagen type I mRNA expression was increased by the addition of up to 10µM orthosilicic acid, ALP message was suppressed over time and osteocalcin levels were decreased.

Publisher

Trans Tech Publications, Ltd.

Subject

Mechanical Engineering,Mechanics of Materials,General Materials Science

Link

https://www.scientific.net/KEM.309-311.121.pdf

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4. M Q Arumugam et al., Orthosilicic Acid Increases Collagen Type I mRNA Expression in Human Bone-Derived Osteoblasts In Vitro, Key Eng. Mat. 254-2, pp.869-872, (2004).

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