Affiliation:
1. Huazhong University of Science and Technology
2. Wuhan University of Technology
Abstract
A novel porous composite scaffold of nano-HA/poly (lactic-co-glycolic) (PLGA) was
fabricated by solvent casting/particulate leaching method. Chondrocytes were isolated from the
knee articular joints of a rabbit, and then seeded in the scaffolds. The cell-loaded scaffolds were
cultured in vitro for 5 days before implantation. Full-thickness articular cartilage defects were
created in rabbits, and filled with and without the cell-loaded nano-HA/PLGA scaffolds. The
implants were harvested after in vivo incubation of 2 and 5 weeks. Cartilaginous tissues were
observed at defects repaired with the cell-loaded scaffolds, while only fibrous tissues were found
for the control groups. The repaired tissues were evaluated histologically by hematoxylin and eosin
staining. Results revealed that nano-HA/PLGA composite scaffolds facilitated adheration of cells in
vitro, and the nano-HA particles could prevented the scaffolds from collapsing and promoted the
formation of cartilaginous tissue in vivo.
Publisher
Trans Tech Publications, Ltd.
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science
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