The Synthesis of Poly-β-Hydroxybutyrate by Moderately Halophilic Bacteria Halomonas venusta

Abstract

In this study, poly-β-hydroxybutyrate (PHB) was synthesized by moderately halophilic bacteria Halomonas venusta and identified by 1H-NMR. The effects of different carbon sources, salt concentration, initial carbon nitrogen quality ratio, initial phosphate concentration were studied on the synthesis of PHB. The optimum conditions of the PHB synthesis were detemined. Glucose was as carbon source with the concentration of 80 g/L and salt concentration, initial carbon nitrogen ratio, initial phosphate concentration was 3%, 15 and 1.9 g/L respectively. Under the above conditions, a two-phase synthesis system of PHB was constructed. The PHB synthesis amount and yield of cell dry weight was reached up to 1.5 g/L and 69.19 wt.%, respectively. The efficient synthesis of PHB was realized.