A Novel Preconcentration Method for Microchip Based Electrophoresis and its Numerical Simulation-Reference-Cited by-同舟云学术

A Novel Preconcentration Method for Microchip Based Electrophoresis and its Numerical Simulation

Published:2012-02 Issue: Volume:503 Page:216-221
ISSN:1662-9795
Container-title:Key Engineering Materials
language:
Short-container-title:KEM

Author:

Cai Hao Yuan¹,Chen Xing¹,Li Hui¹,Cui Da Fu¹

Affiliation:

1. Chinese Academy of Sciences

Abstract

A novel preconcentration method for efficient DNA sample stacking during sample introduction and injection in microchip electrophoresis is presented in this paper. The method suggests that a pair of sample stacking electrodes is designed after the double-T section. A sample stacking electrical field can be established after sample introduction inside the double-T section, so that the sample stacking procedure can be controlled accurately and efficiently. In order to test this method’s validity, a numerical model is setup using COMSOL Multiphysics software. The simulation result shows that the peak DNA concentration can be increased up to 10 fold by this method. These results demonstrate the great potential for significantly enhance DNA sequencing speed and resolution in microchip electrophoresis.

Publisher

Trans Tech Publications, Ltd.

Subject

Mechanical Engineering,Mechanics of Materials,General Materials Science

Link

https://www.scientific.net/KEM.503.216.pdf

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