Behavior of Osteoclast Cells Response on Dicalcium Phosphate Dihydrate Layer-Coated β-Tricalcium Phosphate Granular

Abstract

The aim of this study is to investigate the behavior of osteoclast cells response on dicalcium phosphate dihydrate (DCPD) layer-coated β-TCP granules. β-TCP granules with 300-600 μm were exposed to acidic calcium phosphate solution for 30 mins in order to get 10 mol% DCPD layer-coated β-TCP granular. DCPD free-coated β-TCP granular had used as control specimen. Both specimens were implant in 9 mm of rat calvarial bone defect for 4 weeks. After 4 weeks, the block section of rat calvarial containing specimen were removed for Tatrate-Resistance Acid Phosphatase (TRAP) analysis. Results of TRAP staining reveal that the number of osteoclast cells attached on 10 mol% layer-coated β-TCP granular is higher than DCPD free-coated β-TCP granular. Since remodeling of new bone formation involved simultaneous osteoclast and osteoblast cells response, therefore, the results obtained in this study indicated that the presence of DCPD layer-coated on β-TCP granular helps to improve osteoclast cells response that contribute in stimulating new bone formation.