Effect of Glutaraldehid on Human Amniotic Membrane Characteristics as Wound Dressing

Abstract

The wound dressing which have good mechanical properties, non toxic, anti inflammation, anti infection and has potency to accelerate wound healing are important to be found. This study aimed to determine the effect of glutaraldehyde on the human amniotic membrane cross linking, tensile strength, material toxicity and tissue response to a biomaterial which could be applied as an ideal wound dressing. Human amniotic membrane used was derived from one individual and was obtained from the Biomaterial Center and Tissue Bank in Dr. Soetomo General Hospital Surabaya Indonesia while the glutaraldehyde used was a commercial product. The wound dressing from human amniotic membrane-glutaraldehyde was made by dipping the human amniotic membrane with a solution of different variation of glutaraldehyde compositions, ie 0:25%, 0.5%, 0.75%, 1%, and 1.25%. The results of the research also found the pH measurements on human amnion membrane-glutaraldehyde according to skin balance range (4.0-7.0) by adding 0.25%, 0.5%, 0.75%, 1%, and 1.25% glutaraldehid to human amniotic membrane. FTIR characterization test results indicate the change of intensity of tertiary amine group (C-N) in the human amniotic membrane–glutaraldehyde by 0,25%, 0,5%, 0.75%, 1%, and 1.25% glutaraldehyde concentration. The best concentration of glutaraldehyde to be applied as wound dressing was at a concentration of 1%, which was based on the results of tensile test 26.67 MPa, percentage of living cell in the cytotoxicity assay of 113.483%, and Histopathology Anatomy test that shows 100% of wound re-epithelialization on the mice’s skins.