Development and Characterization of Bilayer Wound Healing Patch Nanofiber Fabricated by Electrospinning

Abstract

The present study aims to develop bilayer polymeric nanofiber patch (PNP) fabricated by electrospinning technique using for wound dressing. The nanofiber was prepared by various concentrations of polyvinyl alcohol (PVA) and modified tamarind seed gum loaded with clindamycin HCl (CM) in first layer and Eudragit® S100 for a second layer. ​According to the SEM result, the physical appearance, the sprayed products prepared from PVA, mixture of PVA and gum, Eudragit® S100 were in round fiber with different diameter size ranged from 153-1830 nm. The polymer concentration, solution conductivity and surface tension affected on the appearance of the nanofiber. The patch was successfully prepared in form of two layer welded with the nanofiber under the optimized electrospinning condition which are 20 kV of applied voltage, 20 cm of injection distance, 0.25 mL of solution feed rate and 48 rpm of collector rolling rate. The DSC and PXRD indicated that the drug in PNPs was in amorphous form. Biological test revealed that bilayer PNPs contained PVA 10%, modified tamarind seed gum 5% and clindamycin 1% had an efficiency to inhibit Staphylococcus aureus. These results show the possibility of improving nanofiber patch strength by using Eudragit® S100 and modified tamarind gum seed as a natural material in nanofiber patch formulation.